Pathogen inducible expression of
S. tuberosum
CyP in local, treated leaves (
upper panel
). RNA was isolated from potato leaves sprayed with zoospores of
P. infestans
at the time points as indicated. mRNA accumulation reached a maximum at12 h. Equal amounts of RNA was separated on agarose gels(rRNA,
middle panel
), blotted and hybridized with a labelled probe derived from the CyP cDNA clone. The expression pattern in non-treated systemic leaves (
lower panel
) only showa comparative slow, weak response.