. Inoculations with Magnaporthe grisea race
007 were carried out as described by Jarosch et al. (1999). Wheat (Triticum
aestivum) powdery mildew elicitor kindly donated by Patrick Schweizer
(IPK Gatersleben, Department of Cytogenetics, Germany; Schweizer et al.,
2000) was sprayed on to 8-d-old barley seedlings at a concentration of 10 g
mL1 Glc equivalents in 10 mm sodium phosphate buffer, pH 7.5, and 0.1%
(w/v) Tween 20. Paraquat (methyl viologen; Sigma, St. Louis) was sprayed
on to primary leaves of 8-d-old barley plants at a concentration of 50 m in
10 mm sodium phosphate buffer, pH 7.5, and 0.1% (w/v) Tween 20, the
plants were incubated in the dark for 2 h, and they were then transferred to
light conditions (150 mol photon m2 s1
). Wounding was carried out by
making transverse cuts with a scalpel blade on primary leaves of 8-d-old
seedlings, and the leaves were then floated on water.
. Inoculations with Magnaporthe grisea race007 were carried out as described by Jarosch et al. (1999). Wheat (Triticumaestivum) powdery mildew elicitor kindly donated by Patrick Schweizer(IPK Gatersleben, Department of Cytogenetics, Germany; Schweizer et al.,2000) was sprayed on to 8-d-old barley seedlings at a concentration of 10 gmL1 Glc equivalents in 10 mm sodium phosphate buffer, pH 7.5, and 0.1%(w/v) Tween 20. Paraquat (methyl viologen; Sigma, St. Louis) was sprayedon to primary leaves of 8-d-old barley plants at a concentration of 50 m in10 mm sodium phosphate buffer, pH 7.5, and 0.1% (w/v) Tween 20, theplants were incubated in the dark for 2 h, and they were then transferred tolight conditions (150 mol photon m2 s1). Wounding was carried out bymaking transverse cuts with a scalpel blade on primary leaves of 8-d-oldseedlings, and the leaves were then floated on water.
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