with greater BFT (Russo et al., 2002). A new PCRRFLP
assay was designed to genotype this SNP (Table
3). The CTSF DNA marker is a missense mutation
identified in exon 9 (D355E; g.22C>G, EMBL accession
numbers AM933487 and AM933486, respectively)
in a highly conserved region of the protein. This mutation
was genotyped by PCR-RFLP as already reported
(Russo et al., 2004; Table 3).