2.5. Chemical analyses
Soil pH was measured in a 2:5 (w/v) ratio of soil to distilled
water (Fuentes et al., 2007). The soil redox potential was
determined using a Pt electrode inserted 10 cm below the soil
surface. Also, the total carbon and total nitrogen in the soil and
plants were determined using a dry combustion CNS analyzer
(LECO CNS-2000).
Approximately 1 g of powdered soil was digested in a 10 ml
solution (1:3) (v/v) (HNO3 (65%)/HCl (37%)) at 150 C for 2 h on a
hot plate. The sample volume was increased to 40 ml with distilled
water. Approximately 0.1 g of the plant sample was added to 2 ml of
HNO3 (65%) before digestion on a hot plate for 3 h at 125 C. The TM
(i.e., Cu, Zn, Cd and Pb) and mineral element (i.e., P, K, Mg and Mn)
concentrations were determined using inductively coupled
plasma mass spectroscopy (ICP-MS). The obtained values are in
accordance (within 5%) with the certified concentrations in the
standard references (calcareous loam soil n141R from BCR in
Belgium, and Virginia tobacco leaves “CTA-VTL-2” in Poland),
confirming the reliability of our analytical procedure.
2.5. Chemical analysesSoil pH was measured in a 2:5 (w/v) ratio of soil to distilledwater (Fuentes et al., 2007). The soil redox potential wasdetermined using a Pt electrode inserted 10 cm below the soilsurface. Also, the total carbon and total nitrogen in the soil andplants were determined using a dry combustion CNS analyzer(LECO CNS-2000).Approximately 1 g of powdered soil was digested in a 10 mlsolution (1:3) (v/v) (HNO3 (65%)/HCl (37%)) at 150 C for 2 h on ahot plate. The sample volume was increased to 40 ml with distilledwater. Approximately 0.1 g of the plant sample was added to 2 ml ofHNO3 (65%) before digestion on a hot plate for 3 h at 125 C. The TM(i.e., Cu, Zn, Cd and Pb) and mineral element (i.e., P, K, Mg and Mn)concentrations were determined using inductively coupledplasma mass spectroscopy (ICP-MS). The obtained values are inaccordance (within 5%) with the certified concentrations in thestandard references (calcareous loam soil n141R from BCR inBelgium, and Virginia tobacco leaves “CTA-VTL-2” in Poland),confirming the reliability of our analytical procedure.
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