Rotary evaporator used
to evaporate the solvent mixture in the extraction of the fat was
R-124 rotavapor with a B-480 Waterbath from Büchi (Switzer-
land). Several borosilicate glass columns of 200 ? 6 mm i.d. with
glass wool on the bottom were filled in the laboratory with the
appropriate amount of sulphuric acid-impregnated silica gel
(SAISG). An ultrasonic bath Branson 3510 (Branson Ultrasonic Cor-
poration, USA) was used in the extraction of the fat. A SPE vacuum
manifold from Waters (MA, USA) with 20 ml reservoirs was also
used.