In conclusion, for the first time we have highlighted the relevance of SIO in both preventive and curative treatments against staphylococcal skin adherence in vitro. Our results tend to support the traditional use of undiluted SIO in skin care. Bioguided fractionation and identification of the most-effective anti-adherent fatty acid could therefore lead to new safe and cost-effective strategies for the treatment of, or prophylaxis against, certain types of skin infections. Omega 3–6 could be responsible, at least partly, for the detachment effect detected on human skin explant, but further experiments are obviously necessary to confirm this hypothesis and also to determinate the best omega 3–6 ratio leading to the best anti-adherence effect.