Cassava stems showing anthracnose disease symptoms (dark brown lesions, deep cankers) were collected from infected field plots at the International Institute of Tropical Agriculture,
Ibadan, Nigeria.
Small pieces of infected materials were cut from the edges of the cankers,
surface-sterilized for 3 min in 10% sodium hypochlorite solution and rinsed five times in sterile distilled water.
The stem pieces were dried on sterilized filter paper and placed on potato dextrose agar (PDA) containing 100 mg/l sodium novobiocin (antibiotic) to inhibit bacterial growth.
The material was incubated at 25° C. Identification of the fungus was carried out under the microscope and confirmation made using the procedure of Barnett and Hunter (1972).
Fungal isolates were determined according to Fokunang et al.(2000b).