To investigation this study, 22 Sites were chosen as clear in Table 1, the first
two Sites lei before Al-Kufa Dam, second four Sites there is no anthropogenic effects
(just some agriculture activity), in the third eight Sites there is direct effect of human
activity with tourism relaxation wastes, in the fourth three Sites the effect of
municipal wastewater of Al-Kufa city and water buffalo are clear, while the last five
Sites affected directly with Al-Brrakia domestic wastewater plant and a lot of buffalo
cultures.