2.2. Estimation of protein denaturation
In this study, temperature is considered to be the main cause of
the protein denaturation of fish flesh during cooking.
The concentration of non-denatured protein (C) (mol g1) with
time could be calculated with the reaction kinetics equation, Eq.
(6), in which k0 is the rate of denaturation constant (min1) and t
is time (s) .