Mfat1 gene insertion analysis
Genomic DNA was extracted from tissue or blood of
the cloned calves named fat1 1-20 using Genomic
DNA extraction Kit. Primers were designed to analyze
the insertion of the mfat1 gene (P1, 50-GGACCTGGT
GAAGAGCATCCG-30; P2,50 -GCGTCGCAGAAGC
CAAAC-30, corresponding to 210–230 and 630–
647 bp of C. elegans fat1 gene, respectively).