Taking into account the results obtained for the leaves, it is
necessary to stress the differences in terms of the number of
differential spots when comparing the previously reported
results for seeds [12] and those for leaves. In our previouswork,
only four protein species were found to be differentially
abundant in seeds (two higher abundant in T seeds and two
others in NT seeds). However, in the present study, a significant
number of protein specieswere found to vary, using a regulation
factor of 1.8 (or 90% variation). In fact, 47 protein species were
found to be differentially abundant (19 higher and 28 lower
abundant). Statistical information from t-tests and the volume
average ratio of these spots are described in Table 2. From this
table, it is clear that all values obtained in the t-test can be
considered significant at p < 0.05 [34]. The volume average ratio
also reveals the extreme differences between protein species in
T and NT soybean leaves.