The influence of five technological parameters selected amongst uncooked semi-hard cheese-making
practices, i. e. milk maturation temperature and time, stirring during maturation, curd stirring time and
pressing time was examined on Staphylococcus aureus growth, enterotoxin gene expression and
enterotoxin (SE) production during cheese manufacture. A fractional factorial experimental design was
applied to perform 32 cheese batches independently inoculated at 103 cfu/ml of milk using four strains
producing SEA, SEB, SEC or SED