medium supplemented with 6-benzylaminopurine (BAP) (0.5–2.0 mg/l) and 1-naphthaleneacetic acid (NAA) (0.1–1.0 mg/l) and agar (0.8%) was used for shoot proliferation. After 4–5 weeks of culture period, the in vitro grown plantlets with newly form shoots were taken out aseptically and the shoots were excised from the parent plant with the help of sterile scalpel blade and forceps and inoculated into new bottles containing solid MS medium with different set of growth regulators (PGR) as mentioned earlier.