Lactic acid was determined by HPLC. Briefly, samples of traditional
and yoba mutandabota were deproteinated by adding 0.25 mL cold
Carrez A solution (42.2 g K4Fe(CN)6·3H2O per 1 L demineralized
water) (Sigma-Aldrich, Steinheim, Germany) to 0.5 mL sample of traditional
or yoba mutandabota in an Eppendorf tube and mixed, then
0.25 mL of cold Carrez B solution (57.5 g ZnSO4·7H2O per 1 L
demineralized water) (Sigma-Aldrich, Steinheim, Germany) was
added and mixed. The Eppendorf tubes with samples were centrifuged
and the supernatant was taken for HPLC analysis (Ultimate 3000,
Dionex), using an Aminex HPX-87 H 300 × 7.8 mm column with a
pre-column (Biorad). The eluent was 5 mM H2SO4 at a flow rate of 0.6
Lactic acid was determined by HPLC. Briefly, samples of traditionaland yoba mutandabota were deproteinated by adding 0.25 mL coldCarrez A solution (42.2 g K4Fe(CN)6·3H2O per 1 L demineralizedwater) (Sigma-Aldrich, Steinheim, Germany) to 0.5 mL sample of traditionalor yoba mutandabota in an Eppendorf tube and mixed, then0.25 mL of cold Carrez B solution (57.5 g ZnSO4·7H2O per 1 Ldemineralized water) (Sigma-Aldrich, Steinheim, Germany) wasadded and mixed. The Eppendorf tubes with samples were centrifugedand the supernatant was taken for HPLC analysis (Ultimate 3000,Dionex), using an Aminex HPX-87 H 300 × 7.8 mm column with apre-column (Biorad). The eluent was 5 mM H2SO4 at a flow rate of 0.6
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