Overall, 2 480 cattle (1 241 in Oyo; 1 239 in Lagos) were screened. Analysis using RBT revealed a total sero-prevalence of 4.9% (121/2 480), with 7.8% and 1.9% from Oyo and Lagos States respectively. The cELISA result supported 77.7% (94/121) (90.7% in Oyo; 25.0% in Lagos) of the total RBT positive samples.