Based on the procedure of Lee et al. [15], the ground powder of the lyophilised sample (30 g) was extracted with 300 mL of 80% (v/v) methanol for 16 h at room temperature with continuous shaking. The
extracts were filtered through Whatman No.1 paper, concentrated
under vacuum at 40°C and freeze-dried. The lyophilized extracts were
stored at 4 o C and, before use; the extracts were dissolved in methanol.