4 cm (350 cm3) jam bottles and then mixed thoroughly with crude
oil concentrations (1, 2.5, 5, 10, 20 and 40%). 20 g of clean rice
straw were laid on the contaminated soil in each bottle, covered
with aluminium foil and autoclaved at 15 lbs pressure for 15 min.
each bottle was then inoculated with two agar plugs of a vigorously
– growing mycelium of L. subnudus using a 7 mm sterile cork borer.
the bottles were incubated at room temperature for 3 – 6 months.
In the first set of control treatments, crude oil was not added to
the soils while in the second set, different levels of oil were added
to all soils but not inoculated with the fungus. At 3 and 6 months
after incubation, the mycelial-ramified waste was separated from
the soils which were analyzed for physico-hemical parameters after
air-drying
4 cm (350 cm3) jam bottles and then mixed thoroughly with crudeoil concentrations (1, 2.5, 5, 10, 20 and 40%). 20 g of clean ricestraw were laid on the contaminated soil in each bottle, coveredwith aluminium foil and autoclaved at 15 lbs pressure for 15 min.each bottle was then inoculated with two agar plugs of a vigorously– growing mycelium of L. subnudus using a 7 mm sterile cork borer.the bottles were incubated at room temperature for 3 – 6 months.In the first set of control treatments, crude oil was not added tothe soils while in the second set, different levels of oil were addedto all soils but not inoculated with the fungus. At 3 and 6 monthsafter incubation, the mycelial-ramified waste was separated fromthe soils which were analyzed for physico-hemical parameters afterair-drying
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