Karyotype analysis of golden little barb (Puntius brevis Bleeker, 1850) from Khon Kaen
province was done in the present study. Mitotic chromosome was prepared directly from kidney
of specimens after In vivo colchicines treatment. Conventional staining and Ag-NOR banding
techniques were applied to stain the chromosomes accomplished by 20% Giemsa’s and 50%
silver nitrate solution, respectively. The results showed that the number of diploid chromosome
of golden little barb was 2n=50, the fundamental numbers (NF) was 62 in both male and
female. The types of chromosomes consists of 4 large metacentric, 4 large submetacentric, 2
large acrocentric, 2 medium acrocentric, 26 meduim telocentric and 12 small telocentric
chromosomes. Ag-NOR banding techniques demonstrated that there is 1 pair of NOR exists at
telomere region on the short arm of acrocentric chromosome pair 6. The karyotype formula of
golden little barb is as follows: 2n (50) = Lm
4 + Lsm
4 + La
2 + Ma
2 + Mt
26 + St
12