a b s t r a c t
This work examined the simultaneous saccharification and fermentation (SSF) process for the biological
conversion of pulp & paper sludge (PPS) and monosodium glutamate waste liquor (MGWL) into ethanol
using Saccharomyces cerevisiae CICC 1001 in batch condition. At the SSF conditions of sterilization, pH 6.0,
6% TS (total solid), and adding nutrient solution, the highest ethanol yield of 42.5 g L−1 was obtained at
the enzyme loading of 40 A.U. (activity unit) g−1 VSfed (volatile solid); only if the pH value was changed
to 4.5, the ethanol yield reduced by 47%; only if the TS concentration was changed to 3%, the ethanol
yield decreased by 11%; only if the substrates were not sterilized, 5% decrease occurred to the ethanol
production; only if no nutrient solution was added to the reactor, 97% reduction of ethanol yield happened;
the extent of influence of the impact factors ranked in the order of nutrient solution > pH > TS
concentration > sterilization. The Fourier Transform Infrared (FTIR) spectra of substrates after SSF further
verified the results of process performances. Therefore, this SSF of PPS and MGWL reduced the residue
streams and land-filling costs while benefiting in ethanol production.
a b s t r a c tThis work examined the simultaneous saccharification and fermentation (SSF) process for the biologicalconversion of pulp & paper sludge (PPS) and monosodium glutamate waste liquor (MGWL) into ethanolusing Saccharomyces cerevisiae CICC 1001 in batch condition. At the SSF conditions of sterilization, pH 6.0,6% TS (total solid), and adding nutrient solution, the highest ethanol yield of 42.5 g L−1 was obtained atthe enzyme loading of 40 A.U. (activity unit) g−1 VSfed (volatile solid); only if the pH value was changedto 4.5, the ethanol yield reduced by 47%; only if the TS concentration was changed to 3%, the ethanolyield decreased by 11%; only if the substrates were not sterilized, 5% decrease occurred to the ethanolproduction; only if no nutrient solution was added to the reactor, 97% reduction of ethanol yield happened;the extent of influence of the impact factors ranked in the order of nutrient solution > pH > TSconcentration > sterilization. The Fourier Transform Infrared (FTIR) spectra of substrates after SSF furtherverified the results of process performances. Therefore, this SSF of PPS and MGWL reduced the residuestreams and land-filling costs while benefiting in ethanol production.
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