Wet gluten quantity (wet gluten yield) was determined based on dough hand washing with tap water and weighing the obtained mass. Dough made from 25.0 g of flour and 12.5 ml of tap water was formed and soaked for 20 min in tap water at room temperature. The dough was hand-washed with tap water until the removal of all the starch (iodine test). Gluten was centrifuged to remove the excess of water. Gluten quality (spreadability) was calculated as the difference between 5 g wet gluten diameters before and after incubation for 60 min at 30 °C, 85% relative humidity (RH).