Leaves and stems, 5 kg each, were air-dried and milled to coarse powder. Both powders were separately macerated exhaustively with dichloromethane (DC) and methanol (MeOH), respectively, for 4 weeks. The supernatants were filtered through Whatman No.1 filter paper. Solvents were evaporated from the filtrate by rotary vacuum evaporator (Buchi, R114, Switzerland). The residues were taken to dryness to obtain a viscous mass as the crude extract. The yield of the dichloromethane crude extracts of leave (LD) and stem (SD) were 5.49 and 0.66% dry weight, respectively. The methanol crude extracts of leaves (LM) and stem (SM) yields were also determined and found 6.52 and 4.62% dry weight, respectively.
Dried-ripe fruits of Z. limonella were blended by a domestic blender to get fine powdered. Essential oil (EO) was obtained by hydrodistillation using a Clevenger-type apparatus, resulting in light yellow oil with 11.63% yield. Essential oil was stored at 4°C until used.