2.4. Determination of bioethanol
Concentration of ethanol in the distillate of culture filtrate was
estimated by using a Hewlett Packard 5890 Series II gas chromatography
with chromosorb 105 column and nitrogen as a
carrier gas. The temperature of the injection port, oven and
detection port was 250, 120, and 250 C, respectively. For
the analysis, 1 ll of liquid samples was injected into gas chromatography.
The ethanol concentration was determined by
using an ethanol standard plot and is expressed in percentage.