The homogenate was centrifuged at 15000 ×g, at 4 °C for 30 min, the lipid layer on the surface was removed, and the supernatant was kept at −20 °C until the assaying of digestive enzymes.
The homogenate was centrifuged at 15000 ×g, at 4 °C for30 min, the lipid layer on the surface was removed, and the supernatantwas kept at −20 °C until the assaying of digestive enzymes.