Cell-cell and cell-matrix interaction depends on the adhesion molecules, which in turn,
relies on the presence of calcium. Tissue culture media contains calcium and
magnesium ions, fetal bovine serum contains proteins that are trypsin inhibitors. Both
Mg2+ and Ca2+ inhibit trypsin. To weaken the function of adhesion molecules, EDTA is
frequently included in the trypsin solution for its function as divalent cations chelator. To
reduce the concentration of divalent cations and proteins that inhibit trypsin action,
before splitting cells with trypsin, the cells should be washed with PBS without
Cell-cell and cell-matrix interaction depends on the adhesion molecules, which in turn, relies on the presence of calcium. Tissue culture media contains calcium and magnesium ions, fetal bovine serum contains proteins that are trypsin inhibitors. Both Mg2+ and Ca2+ inhibit trypsin. To weaken the function of adhesion molecules, EDTA is frequently included in the trypsin solution for its function as divalent cations chelator. To reduce the concentration of divalent cations and proteins that inhibit trypsin action, before splitting cells with trypsin, the cells should be washed with PBS without
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