Before use, the six strains were tested for antagonism among
them, using the spot on-the-lawn bioassay (Lewus & Montville,
1991), adapted to Listeria spp (Bruhn, Vogel, & Gram, 2005).
Briefly, the strains were grown separately overnight in Tryptic Soya
Broth (TSB - Oxoid, Basingstoke, UK) at 37 C, diluted to
107 CFU mL1 in 0.1% sterile peptone water (PW), and an aliquot of
0.1 mL was transferred to a petri dish containing liquid TSB plus 1%
microbiological agar. After mixing and solidification of the agar,
drops of 10 mL of each culture were spotted on the surface of the
agar plates containing each L. monocytogenes culture separately.
Plates were incubated at 37 C for 24 h and examined for growth
inhibition zones around the spots, indicating antagonism and a
reason for exclusion of the strain from the study
Before use, the six strains were tested for antagonism amongthem, using the spot on-the-lawn bioassay (Lewus & Montville,1991), adapted to Listeria spp (Bruhn, Vogel, & Gram, 2005).Briefly, the strains were grown separately overnight in Tryptic SoyaBroth (TSB - Oxoid, Basingstoke, UK) at 37 C, diluted to107 CFU mL1 in 0.1% sterile peptone water (PW), and an aliquot of0.1 mL was transferred to a petri dish containing liquid TSB plus 1%microbiological agar. After mixing and solidification of the agar,drops of 10 mL of each culture were spotted on the surface of theagar plates containing each L. monocytogenes culture separately.Plates were incubated at 37 C for 24 h and examined for growthinhibition zones around the spots, indicating antagonism and areason for exclusion of the strain from the study
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