According to the experiments carried on in vitro by Edgerton [12], yeasts and C. albicans were able to
firmly attach to the surface of resin, glass, ceramics, and metal. At present, the disinfectants chlorhexidine
and formalin, commonly used in cleaning the denture, are effective and inexpensive [13]. However, it is
suggested that they may cause side effects, including staining, bleaching, and being accompanied by
unpleasant odor [14]. In contrast, the antifungal agents tested in this experiment are highly secure,
especially essential oils, which have no residue after treatment.
Butyl paraben sodium and ethyl paraben sodium and potassium sorbate are the most soluble forms of
paraben and sorbate, respectively, and are well-known for their potent antifungal activity. In food systems,
they are the most widely used compounds to prevent the growth of molds and thus extend the shelf life of
products [15]. Our experimental results indicate that butyl paraben sodium, ethyl paraben sodium, and
potassium sorbate had higher antifungal activity with MIC values of 0.25-1.0 mg