2.7. Transmission electron microscopy (TEM)
For TEM analysis, the samples of either the individualised cellghosts or the microfibril suspensions, were deposited on carboncoated electron microscope grids and allowed to dry. Some of these specimens were shadowed with tungsten/tantalum alloys before observations and others were observed as such. The electron microscopy was performed with a Philips CM200 CRYO transmission electron microscope, operated at an acceleration voltage of 80 kV, using a low dose electron beam for observation and recording.
2.8. X-ray diffraction
The X-ray diagrams were recorded on a Warhus flat film vacuum X-ray camera mounted on a Philips PW 1720 X-ray generator operated at 20 mA and 30 kV. X-ray measurements were made on films obtained by water evaporation of the suspensions of cellulose microfibril residues.