Chitosan is a cationic natural polymer derived by the deacetyla-tion of chitin, the major component of the shells of crustaceans. Itis a linear binary heteropolysaccharide composed of -1,4-linkedglucosamine and N-acetylglucosamine, and has been proven to bea non-toxic, biodegradable and biocompatible polymer [8]. It has also been shown to have great potential as antimicrobial packaging material owing to its antimicrobial activity [9,10]. The literature has shown that chitosan can produce films with acceptable permeability to gases (CO2and O2) and good mechanical properties[11]. However, their uses are currently limited owing to their hydrophilic character and weak water resistance. Many strategies have been proposed to improve the barrier and mechanical proper-ties of chitosan-based films. To improve water-barrier properties,hydrophobic compounds, such as lipids, are usually included in the polymer matrix. Over recent years different works have studied the use of fatty acids as lipid components to be incorporated in chitosan films to modulate the water vapor barrier [