Briefly, external primers (HAOUTR and HAOUTF) were used for
the amplification of an 1171-bp segment of the HA gene. Vaccinialike
virus strains isolated from the counties of Cordeiro, Cambuci,
Miracema, Aperibé and strain Cantagalo/IOC were included. The
original Cantagalo strain and the vaccinia strain Wyeth-NYBH
were also included as controls, and PCR was conducted using these
primers as previously described