Forty-nine accessions of JCL with almost identical genetic background based on AFLP
analysis were clustered in Group I, but they were clearly separated into 5 subgroups in combination
analysis. When methylation-sensitive data were analyzed together with AFLP data,
higher resolution of the classification in cluster analysis was obtained. Combination analysis
also separated JCL23 (the hybrid from JCL Anna21 and E-L23) into another subgroup. The
results also provided evidence that environmental factors could have some major effects on
the traits of JCL. This could explain the phenotypic variation observed in JCL without nucleotide
polymorphism. From the present study, high genetic similarity (more than 0.99) among
cultivated JCL samples was observed. The results of the present study were in agreement
with Basha and Sujatha (2007) that JCL has low genetic diversity and requires an immediate
widening of its genetic base. Mutation induced by irradiation was able to cause some changes
in nucleotide sequences and the level of DNA methylation in some samples. However, high
polymorphism was still detected among wild and cultivated JCL samples