3.2.5 Plant regeneration and transfer to the greenhouse.
After 8 months of culture, shoots produced roots were separated. The plantlets with 2-3 cm in height, 2-3 leaves and 2-3 roots were removed from bottles. The rooted shoots were washed thoroughly under running tap water and transplanted into plastic pots with charcoal chips. All pots were then transferred to the greenhouse (Figure 3.1). Twenty plantlets were planted in each pot. The survival rate was recorded after 3 months of culture in the greenhouse.