Semen extension
Buck semen was extended as described by Ajala
et al18 in similar study using pawpaw juice
extender. Briefly, the semen was extended by
dispensing 0.1 ml semen into 0.5 ml extender
(D1 to D7) previously kept at 37oC. However,
whenever the volume of semen collected from
the buck was less than 0.7 ml, extension started
from D2 to D7, to ensure that the test extenders
were included. Immediate assessment of semen
motility was done and recorded. Each tube of
extended semen was thereafter kept away from
light and left at room temperature for 6-hour
period for hourly sperm cell motility assessment