F i g u r e 3 .
Chromatogram of “crude” lycophyll (10) obtained using a C30 carotenoid column (see text). Total lycophyll content (including cis and trans geometric isomers) >90% as AUC. All-trans lycophyll, which elutes at 21.139 min (23.6% AUC), was further purified by semipreparative chromatography and its absolute configuration was confirmed by NMR (Figure 5).