Analyses were performed on 5000
cells, which were acquired at a rate of 300 cells s1. Data
were collected in the form of two-parameter side scatter
(granularity) (SSC) and forward scatter (size) (FSC), and
red fluorescence (FL2) dot plots or histograms were made
on a computerised system. Dead cells were estimated as
the percentage of cells with propidium iodide (red-PI fluorescent
cells, PIþ).