FIG2 Profiles of biofilmmatrix and cell wall-anchored proteins isolated from
S. aureus biofilms. (A) Biofilm matrices were extracted from the indicated S.
aureus strains by 1 M NaCl treatment and analyzed by SDS-PAGE. Arrows,
insolublematerials stacked on the well; arrowheads, Eap (60 kDa) and Efb (15
kDa), which were determined by N-terminal amino acid sequencing. (B) Cell
wall-anchored proteins were isolated fromthe indicated S. aureus strains after
lysostaphin treatment and centrifugation. The supernatants were analyzed by
SDS-PAGE. Molecular sizes are given at the left.