Egg sampleswerecollectedatthefarmla

Egg sampleswerecollectedatthefarmlayerimmediatelyafter
egglayingandthenimmediatelytransportedtothelaboratory.The
eggswerebroken,andthealbumenwasseparatedfromtheyolk
with ahouseholdyolkseparator.Foramineextraction,3gofyolk
samples and3gofalbumensampleswereweighedin50-mLpoly-
propylenecentrifugetubes,and7mLoftrichloroaceticacidat
50 gL
1 (50 gL
1 TCA)wasadded.Thetubeswereagitatedfor
10mininaThermolynetype50800orbitalshaker(Barnstead
Thermolyne,Iowa,USA)at225rpmandthencentrifuged(12,100g)
at 4 °C for21mininachilledBeckmanJ2MCcentrifuge(Beckman
CoulterInc.,California,USA).Aftercentrifugation,thesupernatant
was filteredthroughqualitative filterpaper.Theprocedurewas
repeatedtwomoretimeswiththeadditionof7mLand6mLof
50 gL
1 TCAinatotalof20mLofaddedacid.Theresultingextracts
werestoredin0.5mLmicrotubesandfrozenat
20 °C forlater
derivatizationandchromatographicanalysis.

ผลลัพธ์ (ไทย) 1:

ไข่ sampleswerecollectedatthefarmlayerimmediatelyafteregglayingandthenimmediatelytransportedtothelaboratory การeggswerebroken, andthealbumenwasseparatedfromtheyolkกับ ahouseholdyolkseparator Foramineextraction, 3gofyolkตัวอย่าง and3gofalbumensampleswereweighedin50-mLpoly -propylenecentrifugetubes, and7mLoftrichloroaceticacidat50 gL 1 (50 gL 1 TCA) wasadded Thetubeswereagitatedfor10mininaThermolynetype50800orbitalshaker (BarnsteadThermolyne,Iowa,USA)at225rpmandthencentrifuged(12,100g)ที่ 4 ° C for21mininachilledBeckmanJ2MCcentrifuge (BeckmanCoulterInc. แคลิฟอร์เนีย สหรัฐอเมริกา) Aftercentrifugation, thesupernatantถูก filteredthroughqualitative filterpaper Theprocedurewasrepeatedtwomoretimeswiththeadditionof7mLand6mLof50 gL 1 TCAinatotalof20mLofaddedacid.Theresultingextractswerestoredin0.5mLmicrotubesandfrozenat 20 ° C forlaterderivatizationandchromatographicanalysis

ผลลัพธ์ (ไทย) 2:

ไข่ ahouseholdyolkseparator.Foramineextraction, 3gofyolk ตัวอย่าง and3gofalbumensampleswereweighedin50-mLpoly- propylenecentrifugetubes, and7mLoftrichloroaceticacidat 50 แอล 1 (50 แอล 1 4 องศาเซลเซียส filteredthroughqualitative filterpaper.Theprocedurewas repeatedtwomoretimeswiththeadditionof7mLand6mLof 50 แอล 1 TCAinatotalof20mLofaddedacid.Theresultingextracts werestoredin0.5mLmicrotubesandfrozenat? 20 ° C forlater derivatizationandchromatographicanalysis

ผลลัพธ์ (ไทย) 3:

sampleswerecollectedatthefarmlayerimmediatelyafter ไข่egglayingandthenimmediatelytransportedtothelaboratory .eggswerebroken andthealbumenwasseparatedfromtheyolk ,กับ ahouseholdyolkseparator foramineextraction 3gofyolk , .and3gofalbumensampleswereweighedin50 mlpoly - ตัวอย่างpropylenecentrifugetubes and7mloftrichloroaceticacidat ,50 gl1 ( 50 gl1 TCA ) wasadded.thetubeswereagitatedfor10mininathermolynetype50800orbitalshaker ( บาร์นสเต็ดthermolyne , Iowa , USA ) at225rpmandthencentrifuged ( 12100g )ที่ 4 ° C ( for21mininachilledbeckmanj2mccentrifuge เบคแมนcoulterinc , California , USA ) aftercentrifugation thesupernatant ,คือ filteredthroughqualitative theprocedurewas filterpaper .repeatedtwomoretimeswiththeadditionof7mland6mlof50 gl1 theresultingextracts tcainatotalof20mlofaddedacid .werestoredin0.5mlmicrotubesandfrozenat 20 ° C forlaterderivatizationandchromatographicanalysis .