Our results showed that the untreated HCT116 cells (i.e.,
the control group) emitted blue fluorescence DAPI with a constant
intensity, representing the homogeneity of chromatin
distribution in the nucleus and indicating no DNA fragmentation
and non-apoptotic cells (Fig. 1). Three positive control
groups (viz., mitomycin C-, sulforaphane- and sulforaphenetreated
HCT116 cells) and the Thai rat-tailed radish extracttreated
HCT116 cells displayed different nuclear morphologies
from the untreated cells; as seen by chromatin condensation
and nuclear fragmentation, indicating the occurrence of
apoptotic processes (Fig. 1).