Two submersible aquarium pumps (Quiet1one 1200, Aquasonic,
Wauchope, NSW, Australia) were connected to each respirometer.
One pump recirculated water inside the respirometer at a rate of 1.0 exchange min
−1
to ensure proper mixing inside the chamber. The other
pump was connected to a digital timer (DRT-1, Sentinel, China), which
intermittently exchanged water inside the respirometer at a rate of
1.0 exchange min
−1
with seawater from the ambient tank for 10 min
every 20 min, creating a 10-min closed and a 10-minflush cycle. This
allowed a
_ MO2measurement every 20 min.
Two submersible aquarium pumps (Quiet1one 1200, Aquasonic,Wauchope, NSW, Australia) were connected to each respirometer.One pump recirculated water inside the respirometer at a rate of 1.0 exchange min−1to ensure proper mixing inside the chamber. The otherpump was connected to a digital timer (DRT-1, Sentinel, China), whichintermittently exchanged water inside the respirometer at a rate of1.0 exchange min−1with seawater from the ambient tank for 10 minevery 20 min, creating a 10-min closed and a 10-minflush cycle. Thisallowed a_ MO2measurement every 20 min.
การแปล กรุณารอสักครู่..