For isolation of Shigella spp., conventional culture
method described by Food and Drug Administration
(FDA) was used (Andrews & Jacobson, 2000). Samples
(25 g or ml) were homogenized by means of a seward stomacher,
model 400 circulator ( 2 min) in 225 ml shigella
broth in which novobiocin (Sigma) was added (0.5 lg/ml
for S. sonnei and 3.0 lg/ml for other Shigella spp.). It
should be noted that, for chicken samples 25 g sample
was taken from skin and muscle tissues of multiple points
on chicken parts. The homogenized samples were then