In another experiment, we compared the speed of kill (median
survival time (ST50)) using blastospores from ESAL
Q1432 also produced in media containing 40, 100, or 140 g
glucose L−1. Blastospores from each glucose treatment were
tested on four replicate leaves. A single-dosage of 2.7×104
blastospores cm−2 was used for each treatment and then applied
to infested leaves and monitored daily throughout 6 days
incubation for mortality. This single-dosage experiment was
conducted three times using different insect cohorts and fungal
batches. Viability of blastospores were assessed before
spraying and resulted in 70–80 % germination in all cases,
after 7 h incubation in PD broth at 28 °C and 300 rpm