and mixed, then
0.25 mL of cold Carrez B solution (57.5 g ZnSO4·7H2O per 1 L
demineralized water) (Sigma-Aldrich, Steinheim, Germany) was
added and mixed. The Eppendorf tubes with samples were centrifuged
and the supernatant was taken for HPLC analysis (Ultimate 3000,
Dionex), using an Aminex HPX-87 H 300 × 7.8 mm column with a
pre-column (Biorad). The eluent was 5 mM H2SO4 at a flow rate of 0.6
Fig. 1. Flow chart for the production of yoba mutandabota.
44 A. Mpofu et al. / International Journal of Food Microbiology 217 (2016) 42–48
ml/min at 40 °C. Detection was done by refractive index (Shodex RI
101). Sample volume was 10 μL and the run time was 30 min.