A loop of H. palleronii strain was initially inoculated into 50 ml
of nutrient broth in 500 ml flasks, and kept in shaking incubator
under dark condition at 30 C for overnight at 180 rpm. For growth
experiments 4 ml (4% v/v) of the overnight grown culture was
inoculated into different shake flasks containing 100 ml of SW with
different carbon concentrations i.e., 5–60 g/l. The experiments
were conducted for 192 h. Samples were collected at different time
intervals, and growth was monitored spectrometrically by measuring
the absorbance at 600 nm using UV-spectrometer (UV-1800,
Shimadzu, Japan).