Raw material and sauce formulations: Sardines (Sardina pilchardus) caught from Aegean Sea in Turkey. Fish samples were transported to the laboratory with ice packs within 2 h after landing. They were gutted, sorted, prepared as a fillet and washed with fresh water. Approximately 45 kg of sardines were used for this study and shared in 6 groups for different formulations of sauces. Each group was about 5 kg. Laboratory fish sauce production all sardines fillets were cut in to pieces about 1 cm long. On the other hand, at the same time formulations of additives were prepared. The mixture of group A was 10 g NaCl and 100 g fish for each bottle of this group. The formulation of group B was 10 g NaCl + 1 g red pepper + 1 g garlic powder + 100 g fish samples. Group C performed with 10 g NaCl + 5 g glucose + 100 g fish sample. Group D was 10 g NaCl + 5 g glucose + 1 g red pepper + 1 g garlic powder + 100 g fish sample. In group E 10 g NaCl + 10 g glucose + 100 g fish were used. And for the last formulation, group F was prepared with 10 g NaCl + 10 g glucose + 1 g red pepper + 1 g garlic powder + 100 g sardine samples. The ratios were calculated according to these formulas and used in each 5 kg samples pools. For each group 70 and totally 420 bottles were used in the study. Each bottle was filled with mixtures by hand, using plastic gloves. The capacity of the glass bottle was 200 mL, the color was dark brown. The tips of the bottles were plastic and no air could enter when they were closed. When all bottles were filled and closed they were incubated at 37°C in an incubator for 57 days. In the last day of fermentation, 6 bottles were taken for amino acid determination analysis and for determining fatty acid composition.