Therefore, they have the
potential to be used as medicinal herbs or as animal
hormonal feed supplements. To investigate the
toxicity of the plants on humans and animals before
consumption, one useful and highly sensitive
screening test is to expose the plant extract to the
early life stage of zebrafish (Danio rerio) or the fish
embryonic toxicity test which is established (OECD
1992; ISO 1999; Carlsson and Norrgren, 2004). The test
is one of the most widely used tools in environmental
science research, especially for investigating the
toxicity and teratogenicity of chemicals that could
significantly affect environmental health (Xiaoshan et
al., 2007). In many studies, D. rerio has been used as a
vertebrate model to study the developmental toxicity
of chemical compounds and has contributed to the
understanding of the potential toxicological and
ecotoxicological impact on the human and aquatic
environment