Materials and methods
Materials
Fresh raw buffaloes’ milk was obtained from the herd of the
Faculty of Agriculture, Ain Shams University. Buffalos’ milk
was mechanically separated to skim milk (0.1% fat and
9.5% SNF) for manufacturing Kareish cheese. Commercial
fine grade salt of El-Nasr Salines Company, Egypt and calcium
chloride from Sigma Chemical Company, Str. Louis, USA,
were used.
As coagulant, Hannilase rennet powder (CHY-Max powder
extra) was purchased from Chr. Hansen’s Lab., Denmark.
Two commercial freeze-dried DVS mixed bacterial starters of
CH-1 (containing of Lb. delbrueckii ssp. bulgaricus and Streptococcus
(Str.) thermophilus) as yoghurt starter and ABT-1
(containing of Lb. acidophilus, Str. thermophilus and bifidobacteria)
with potential probiotic properties (from Chr. Hansen
Laboratory, Copenhagen, Denmark) were used in the fermentation
process. Freeze-dried bacterial starters were prepared
separately as mother cultures in autoclaved (121 _C/10 min)
fresh buffaloes’ skim milk using 0.02% (w/v) inoculums. The
cultures were incubated at 42 _C for CH-1 starter and 39 _C
for ABT-1 starter, until curdling of milk. Cultures (108–
109 cfu/ml) were prepared 24 h before use.
Materials and methods
Materials
Fresh raw buffaloes’ milk was obtained from the herd of the
Faculty of Agriculture, Ain Shams University. Buffalos’ milk
was mechanically separated to skim milk (0.1% fat and
9.5% SNF) for manufacturing Kareish cheese. Commercial
fine grade salt of El-Nasr Salines Company, Egypt and calcium
chloride from Sigma Chemical Company, Str. Louis, USA,
were used.
As coagulant, Hannilase rennet powder (CHY-Max powder
extra) was purchased from Chr. Hansen’s Lab., Denmark.
Two commercial freeze-dried DVS mixed bacterial starters of
CH-1 (containing of Lb. delbrueckii ssp. bulgaricus and Streptococcus
(Str.) thermophilus) as yoghurt starter and ABT-1
(containing of Lb. acidophilus, Str. thermophilus and bifidobacteria)
with potential probiotic properties (from Chr. Hansen
Laboratory, Copenhagen, Denmark) were used in the fermentation
process. Freeze-dried bacterial starters were prepared
separately as mother cultures in autoclaved (121 _C/10 min)
fresh buffaloes’ skim milk using 0.02% (w/v) inoculums. The
cultures were incubated at 42 _C for CH-1 starter and 39 _C
for ABT-1 starter, until curdling of milk. Cultures (108–
109 cfu/ml) were prepared 24 h before use.
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