The total anthocyanine was measured using method of Cha et al. (14) with
modification.
The 0.1 g samples were placed in an Erlenmeyer flask with 200 mL of extraction solvent (EthOH:water:HCl=85:13:2), left the mixture for 2 h at room temperature and centrifuged it to extract the upper level liquid as test solution.
The test solution was measured as having an absorbance of 535 nm by spectrophotometer (Optizen 2120 UV; Mecasys, Daejeon, Korea).