Five litter subsamples per chamber were sampled to obtain a
representative complete litter profile (caked and friable). Pooled
litter subsamples were then thoroughly mixed in a 1 L plastic box
and moisture content and pH were assayed. Litter pH was determined by mixing litter and de-ionised water in the ratio of 1:5 and
measuring with a pH meter (EcoScan 5/6 pH meter, Eutech Instrument Pte Ltd; Singapore).