However, the 1.08-kb fragment was absent from AAC00-1ΔhrcC and a smaller 0.4-kb fragment was amplified, indicating deletion of the hrcC gene. As expected, both amplicons were produced with DNA from AAC00-1ΔhrcCcomp (Fig. 1).
However, the1.08-kb fragment was absent from AAC00-1ΔhrcC and a smaller0.4-kb fragment was amplified, indicating deletion of the hrcCgene. As expected, both amplicons were produced with DNA fromAAC00-1ΔhrcCcomp (Fig. 1).