3.2. PCR analysis
A total of 89.3% of the swabs from the tested lungs with
lesions suggestive of EP (143/160) were positive by realtime
PCR for M. hyopneumoniae with a concentration of
bacteria ranging from 1.17 105 to 3.37 109 genomes
per mL. No link was observed between numbers of M.
hyopneumoniae cells and severity of lesions in abattoir pigs
(Fig. S1A). Using multiplex PCR, 10% of the lungs with
lesions suggestive of EP (16/160) were positive for M.
hyorhinis whereas 8.8% (14/160) were positive for M.
flocculare. Two lungs (1.25%) carried a mixture of the three
mycoplasmas whereas no mycoplasmas were detected in
seven lungs (4.4%). None were positive for M. flocculare
without the other two mycoplasmas while seven lungs
(4.4%) were positive for M. hyorhinis alone.
Supplementary material related to this article can be
found, in the online version, at http://dx.doi.org/10.1016/
j.vetmic.2013.11.006.