In this study, cellulose/lignin composite hydrogels in various cellulose to lignin ratios were prepared and used for immobilizing lipase from Candida rugosa. The effects of lignin content in the cellulose/lignin hydrogels on the protein loading, activity, and stability of immobilized lipase were investigated by systematically changing the hydrogel compositions. The controllability and predictability of the properties of hydrogel beads and the activity of immobilized lipase were also determined by statistical analysis.